Fig. 6.

MΦ0 BMDMs from in vivo liraglutide-treated ApoE^−/− mice. BMDMs were taken from ApoE^−/− mice treated with liraglutide in vivo. BMDMs were differentiated into M0 macrophages and analysed for a CCR7, b IL-6, c iNOS, d TNF-alpha, e Arg-1, f IL-10, g MR and h CD163 by RT-qPCR with 18SrRNA as the reference gene. BMDMs were also analysed by flow cytometry for i monocyte and j macrophage populations using antibodies specific against CD45, CD11b, CD11c, F4/80 and Ly-6C. Error bars are representative of 6 mice per group (n = 6) for RT-qPCR and 5 mice per group (n = 5) for monocytes and 4 mice per group (n = 4) for macrophage flow cytometry analysis. Statistical analysis was performed carrying out Mann–Whitney t tests comparing all groups. Statistical significance was considered when *p < 0.05, **p < 0.01 and ***p < 0.001. p > 0.05 was considered NS. Stars above the columns represent comparisons made against the LFD control while capped lines indicate comparisons made between other groups