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. 2017 Nov 6;13:311. doi: 10.1186/s12917-017-1232-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Specificity of the real-time RPA assay for CPV-2 detection. Real-time RPA was carried out at 38 °C for 20 min using 10 ng of viral DNA or cDNA as template. The results showed real-time RPA only amplified the CPV-2a and CPV-2b DNA, but not other viruses tested (n = 5). Lane 1, CPV-2a; lane 2, CPV-2b; lane 3, CDV; lane 4, CCoV; lane 5, CPIV; lane 6, PRV; and lane 7, canine genomic DNA