Figure 5.
A contractile junctional adhesion belt is associated with cell flattening in anaphase. (A) Definition of measured parameters for cell roundness estimation in the x-z dimension. (B and C) MDCK-ΔEcad and MDCK-Par1b cells from Fig. 4 E and Fig. 3 C, respectively, were analyzed for m, h/d, and Cx-z values. Note that control cells (+Dox) are less rounded than ΔEcad or Par1b cells (−Dox). n = 25 cells/experiment were analyzed for N = 3 independent experiments. Error bars indicate +SD. *, P ≤ 0.05; **, P ≤ 0.01; ns, not significant, analyzed by t test. (D and F) MDCK-ΔEcad and MDCK-Par1b cells were fixed and stained as indicated. (F) Red arrowhead shows hepatocytic-like lateral lumina. The P-MLC2 intensity spectrum maps for the x-z views are shown (right). The intensity of P-MLC2 at the apex (white dashed lines) was quantified. White and yellow arrowheads denote junctional activated myosin II in the mitotic cell and its neighbors, respectively. n = 15 cells/experiment were analyzed for N = 3 independent experiments. Error bars indicate +SD. ***, P ≤ 0.001, analyzed by t test. (E and G) Correlation of anaphase P-MLC2 intensity and Cx-z in control (+Dox) and ΔEcad (E) or Par1b (G) cells (−Dox). §§§, P ≤ 0.001.