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. 2017 Nov 6;216(11):3463–3470. doi: 10.1083/jcb.201609114

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© 2017 Leitao and Kellogg

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Figure 2. — Simultaneous imaging of bud growth and mitotic spindle dynamics. (A and B) Representative growth curves for cells growing in rich carbon (A) or poor carbon (B). The volume of the daughter bud is plotted in blue, and the distance between spindle poles in green. (C) Contrast-enhanced images of a representative growing bud with GFP-tagged spindle poles (Spc42-GFP) and myosin ring (Myo1-GFP). Key transitions are highlighted. “…” indicates images taken in metaphase that were omitted so that all key transition points could be shown. Bars, 2 µM.