Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 6;216(11):3713–3728. doi: 10.1083/jcb.201610039

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Pitaval et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Microtubule network remodeling during centrosome migration. (A and B) Microtubule network organization was studied by time-lapse imaging of RPE1 cells expressing EGFP-centrin1 and MAP4-RFP. Two examples are shown: one where the centrosome (indicated by white arrowheads) migrated to the apical pole (A) and another where it did not (B). Orthogonal and top views are shown. Microtubule network symmetry breaking and densification are shown with yellow arrows. (C) Measurement of centrosome z position (left) and α-tubulin fluorescence intensity in a 5-µm box surrounding the centrosome (right) in thymidine-synchronized serum-starved RPE1 cells expressing EGFP-centrin1 for various delays after serum removal (n = 75 cells per condition). Horizontal bars show mean values. Error bars represent standard deviation. (D) The graph shows the microtubule network density at the centrosome against centrosome z position at various time points after serum starvation in thymidine-synchronized RPE1 cells expressing EGFP-centrin1. In all conditions, the two parameters were correlated. (E) RPE1 cells expressing EGFP-centrin1 (centrosome indicated by white arrowheads) were fixed 120 min after serum withdrawal and stained for α-tubulin and DAPI. The side views facilitated the visualization of microtubule bundle orientation and quantification of cell proportion exhibiting either a microtubule bundle toward the basal pole (images and bar graph with yellow outlines), a microtubule bundle toward the apical pole (images and bar graph with green outlines), or no detected microtubule bundle (images and bar graph with red outlines; results of four independent experiments, n = 116 cells). (F) Staining of serum-starved RPE1 cells expressing EGFP-centrin1 with an antibody to EB1. The graph shows EB1 fluorescence intensity measurements in a 5-µm box surrounding the centrosome (one experiment, n = 60 cells per condition). Bars: (x and y) 10 µm; (z) 5 µm. a.u., arbitrary units. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.