Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 6;216(11):3767–3783. doi: 10.1083/jcb.201612006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Wood et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — αCat^KKR<3A mutant alters cell–cell adhesion and sheet migration. (A) Live imaging of αCat-expressing R2/7 cells imaged for 12 h postwounding. Arrows indicate αCat recruitment in FLαCat but not FLαCat^KKR<3A. (B) Quantification of enrichments (n > 150 FOVs inclusive of all time points, two BRs). Data are mean ± SD. (C) Representative image of wound closure area. Pink overlay depicts area quantified. (D) Quantification of wound closure (n = 24 FOVs, two BRs, data are mean ± SD). See corresponding Video 4. (E) Phalloidin stain of F-actin at wound front. Red arrows indicate actin cables parallel to the front; black arrows indicate actin cables adjoining cells via junctions. High-resolution analysis of F-actin across adjacent cells by SIM (right). (F) Live imaging of GFP-LifeAct and mCherry FLαCat during wound migration. (Left) Fluorescent image; white arrows indicate protrusions with long filopodia; yellow arrowheads show areas of colocalization. (Right) Bright-field image. Bars, 50 µm. (G) Representative images from a mechanical disruption assay with quantification in H (Materials and methods; n = 5 BRs). Data are mean ± SD; significance in all panels by unpaired t test: ****, P < 0.0001.