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. 2017 Nov 6;216(11):3535–3549. doi: 10.1083/jcb.201612160

Table 2. sgRNA sequences used for lentiviral vectors and Alt-R crRNA generation.

Gene sgRNA sequences
SETDB1 sgRNA 6 5′-TGGAAGTCCCGAGTTGAGG-3′
SETDB1 sgRNA 9 5′-TGGTGGAAGTCCCGAGTTG-3′
SETDB1 sgRNA 10 5′-CCACTCTTGAGCAGTACCA-3′
NTC (Luciferase) 5′-GCATGCGAGAATCTCACGC-3′
PLK1 5′-GTTGTCCTCGAAAAAGCCG-3′
MAVS sgRNA 1 5′-GGATTCCTTGGGATGGCTC-3′
MAVS sgRNA 2 5′-CTGGAGTCCTCCTCTGACC-3′
MAVS sgRNA 3 5′-CAGCCTCACACCATCCCGT-3′
MAVS sgRNA 4 5′-GAGACACAGGCCCACGGGA-3′
MAVS sgRNA 5 5′-CAGGGTCAGTTGTATCTAC-3′
MDA5 sgRNA 1 5′-TAGCGGAAATTCTCGTCTG-3′
MDA5 sgRNA 2 5′-GCCTGCATGTTCCCGGAGG-3′
MDA5 sgRNA 3 5′-ACTGCCTGCATGTTCCCGG-3′
MDA5 sgRNA 4 5′-CATGAGCGTTCTCAAACGA-3′
MDA5 sgRNA 5 5′-AGAAATGGTATCGTGTTAT-3′
RIG-I sgRNA 1 5′-AGCCTTCCAGGATTATATC-3′
RIG-I sgRNA 2 5′-GATTATATCCGGAAGACCC-3′
RIG-I sgRNA 3 5′-AACAACAAGGGCCCAATGG-3′
RIG-I sgRNA 4 5′-GATCAGAAATGATATCGGT-3′
RIG-I sgRNA 5 5′-ATTTCTGCTGTTCATACAC-3′
CD81 sgRNA 2 (control) 5′-GTTGGCTTCCTGGGCTGCTA-3′