Figure 5.

Alm1 is required for proper accumulation of Cut8 and the proteasome to the NE. (A) WT cells expressing Cut8-GFP and Alm1-Tomato. Images are maximal projections of three z sections. (B) WT (red lectin stained) and alm1Δ cells expressing Cut8-GFP. Cut8-GFP intensity levels at the NE in WT and alm1Δ cells. Graphs represent mean and SD. n = 69. (C) Western blot analysis of total Cut8-GFP protein of WT and alm1Δ cells using anti-GFP mAb to detect Cut8-GFP and anti-PSTAIR mAb as a loading control. (D) Brightfield and immunofluorescence images of WT and alm1Δ cells expressing Mts2-8Myc, using anti-Myc antibodies against Mts2-8Myc (green) and DAPI to stain DNA (blue). (E) Western blot analysis of total Mts2-8Myc protein in WT and alm1Δ cells using anti-Myc antibodies to detect Mts2-8Myc and anti-PSTAIR antibodies as a loading control. (F) Brightfield and immunofluorescence images of WT and alm1Δ cells expressing Mts4-13Myc, using anti-Myc antibodies against Mts4-13Myc (green) and DAPI to stain DNA (blue). (G) Western blot analysis of total Mts4-13Myc protein in WT and alm1Δ cells using anti-Myc antibodies to detect Mts4-13Myc and anti-PSTAIR antibodies as a loading control. Positions of molecular mass markers are indicated in kilodaltons. Bars, 5 µm. A.U., arbitrary units. ***, P < 0.001.