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. 2017 Nov 6;216(11):3521–3534. doi: 10.1083/jcb.201703144

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© 2017 Patel et al.

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Figure 3. — BLM regulates HR in BRCA1^Δ11 cells. (A) Western blot analysis of EJ-DR reporter cells transfected with siRNA oligos and/or BRCA1 expression vector or control empty vector (EV). (B) Representative flow cytometry data in the U2OS EJ-DR reporter assay, in which HR-mediated repair of I-SceI–mediated DSBs produces GFP fluorescence and mutagenic NHEJ (mNHEJ) produces DsRed fluorescence. (C) Analysis of relative HR frequency in the U2OS EJ-DR reporter cell line. (D) Analysis of relative mNHEJ frequency in the U2OS EJ-DR reporter cell line. Graphs represent mean ± SD of three independent experiments.