Figure 1.
Screen for channels linked to the yeast mitochondrial outer membrane. (Lanes 1–3) Mitochondrial outer membrane (OM) vesicles (OMVs) from por1Δ and WT yeast and mitochondria (Mito.) were analyzed by SDS-PAGE and Coomassie staining. (Lanes 4–6) Crude and purified mitochondria and outer membrane vesicles from por1Δ were analyzed by SDS-PAGE and immunodetection. (Lanes 7–11) Proteins were recombinantly expressed, purified, and analyzed by SDS-PAGE and Coomassie staining. IM, inner membrane.