Table 2.
Vertical transmission of MHV68 in ticks.
| Experiment | Ticks | Molted ticks | Treatment | Number of mice tested | Mouse samplea | Number (%) virus positive miceb | Days after virus injection of F0 ticks | ||
|---|---|---|---|---|---|---|---|---|---|
| Nested PCR | qPCR | RT-PCR | |||||||
| 5 | Adults (F0i) | n.a. | Injected with MHV68 and fed on uninfected mice | 20 | Blood | 9 (45%) | n.t. | n.t. | 38 |
| 5 (control) | Adults (F0c) | n.a. | Fed on uninfected mice | 2 | Blood | 0 (0%) | n.t. | n.t. | n.a. |
| 6 | n.a. | Larvae (F1i) | Fed on uninfected mice | 9 | n.t. | n.a. | n.a. | n.a. | 112 |
| 6 (control) | n.a. | Larvae (F1c) | Fed on uninfected mice | 2 | n.t. | n.a. | n.a. | n.a. | n.a. |
| 7 | n.a. | Nymphs (F1i) | Fed on uninfected mice | 9 | Blood | n.t. | 9 (100%) | 7 (77%) | 252 |
| Lung | n.t. | 9 (100%) | 7 (77%) | ||||||
| Spleen | n.t. | 9 (100%) | 0 (0%) | ||||||
| 7 (control) | n.a. | Nymphs (F1c) | Fed on uninfected mice | 2 | Blood | n.t. | 0 (0%) | 0 (0%) | n.a. |
| Lung | n.t. | 0 (0%) | 0 (0%) | ||||||
| Spleen | n.t. | 0 (0%) | 0 (0%) | ||||||
| 8 | n.a. | Adults (F1i) | Fed on uninfected mice | 9 | Blood | 0 (0%) | 9 (100%) | n.d. | 525 |
| Lung | 8 (88%) | 9 (100%) | 7 (77%) | ||||||
| Spleen | 6 (66%) | 9 (100%) | 4 (44%) | ||||||
| 8 (control) | n.a. | Adults (F1c) | Fed on uninfected mice | 1 | Blood | 0(0%) | 0 (0%) | n.d. | n.a. |
| Lung | 0 (0%) | 0 (0%) | 0 (0%) | ||||||
| Spleen | 0 (0%) | 0 (0%) | 0 (0%) | ||||||
n.a., not applicable; n.t., not tested.
a
Samples collected from mice 15 days post-infestation.
b
Nested PCR detects ORF50 gene; qPCR detects ORF65 gene representing genome equivalent number; RT-PCR detects M3 gene transcripts.