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. Author manuscript; available in PMC: 2018 Nov 25.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Sep 27;493(3):1236–1242. doi: 10.1016/j.bbrc.2017.09.134

Figure 2. Effect of TRB3 overexpression on protein synthesis in mouse skeletal muscle.

Figure 2

GAS muscles were collected from WT and TRB3TG mice. (A–D) The activations of signaling molecules related to protein synthesis, including mTOR (A), S6K1 (B), and Akt (C and D), were analyzed by Western blot. (E–G) After 5 hours of fasting, mice received an intraperitoneal injection of 0.04 µmol/g body weight with puromycin dissolved in 100 µl of PBS 30 min before euthanasia to measure the rate of protein synthesis. Western blot was used to measure the amount of puromycin incorporation into nascent peptides. (E) Ponceau S staining of puromycin analysis from SOL muscle showed that proteins were equally loaded. EDL (F) and SOL (G) muscles were analyzed. Data are the means ± S.E.M. (n = 5–6 per group). * P < 0.05, ** P < 0.01, *** P < 0.001 vs. WT