Skip to main content
. Author manuscript; available in PMC: 2017 Nov 7.
Published in final edited form as: Cancer Res. 2010 Sep 22;70(21):8726–8735. doi: 10.1158/0008-5472.CAN-10-0755

Figure 3. The CCAAT box and the NF-Y transcription factor are required for optimal MLL1 activation of MDR1 transcription.

Figure 3

A, MDR1 transcriptional activity was measured 24h post-co-transfection of f-MLL1 and a luciferase reporter construct driven by different deletions or point mutations of the MDR1 promoter. Luciferase activities were measured and normalized to protein concentration and luciferase activities of empty vectors (pGL2B and pCXN2). B, MDR1 transcriptional activity was measured by luciferase activity 24h post-cotransfection of a luciferase reporter construct driven by the MDR1 promoter with empty vector, f-MLL1, a dominant negative NF-Y (NF-Y(DN)), or both. Luciferase activities were measured and normalized to protein concentration and luciferase activities of empty vectors (pGL2B and pCXN2).