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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Cancer Res. 2017 Aug 15;77(17):4736. doi: 10.1158/0008-5472.CAN-17-1255

Fig. 1. YAP1 and TAZ control of TG2 level in breast cancer stem cells.

Fig. 1

MCF-7 cells were double electroporated with Control-, YAP1- or TAZ-siRNA and after 48 h extracts were prepared to monitor the level of the indicated proteins and the extent of spheroid formation as previously described. These studies show that YAP1 knockdown reduces TG2 level, and that TAZ knockdown reduces YAP1, YAP1-P and TG2 level. We performed parallel experiments and showed that YAP1- or TAZ- knockdown reduces MCF-7 cell spheroid formation. Similar results were observed in each of three separate experiments.