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. 2017 Nov 7;12(11):e0179577. doi: 10.1371/journal.pone.0179577

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© 2017 Tran et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A, SPHK1 (red). B, SPHK2 (red). C, Spns2 (red, LifeSpan BioSciences rabbit polyclonal antibody). Green in A-C was beta-actin. D, E, Spns2 (green, Santa Cruz goat polyclonal antibody) was expressed in cilia, and colocalized with SPHK1 (red, yellow being merged color of red and green) in the cytoplasm. F, a negative staining control incubated with conjugated antibodies alone. Blue in A-F was DAPI. Arrows in C, D, E indicate Spns2 expression in cilia. Scale bars are in micrometers. Images are representative of bronchial epithelial cells obtained from 3 different non-smoking donors.