| Escherichia coli |
|
|
| Novablue |
endA1 hsdR17 (rK12–
mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+
lacIqZΔM15::Tn10]
|
our laboratory collection; standard cloning strain |
| DB3.1 |
F-
gyrA462 endA1 glnV44 Δ(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2 rpsL20 xyl5 Δleu mtl1
|
our laboratory collection; CcdB-resistant cloning strain |
| W1872 |
F+ λ–
|
Coli Genetic Stock Center; harbors wildtype F-plasmid |
|
E. coli K-12 MG1655 |
F−λ−ilvG−rfb-50 rph-1
|
our laboratory collection; K-12 wildtype strain |
| BW25113 |
F- Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) λ-
rph-1 Δ(rhaD-rhaB)568 hsdR514
|
our laboratory collection; commonly used lab strain derivative of K-12 MG1655 (deficient in EcoKI restriction) |
| MFDpir |
RP4-2-Tc::[ΔMu1::aac(3)IV-ΔaphA-Δnic35-ΔMu2::zeo] ΔdapA::(erm-pir) ΔrecA
|
Didier Mazel [63]; conjugation donor strain with chromosomal RP4 T4SS (derivative of K-12 MG1655) |
| JKE170 |
MFDpir ΔmcrA Δ(mrr-hsdRMS-mcrBC)
|
this study; derivative of MFDpir lacking all type IV restriction systems |
| JKE201 |
MFDpir ΔmcrA Δ(mrr-hsdRMS-mcrBC) aac(3)IV::lacIq
|
this study; derivative of MFDpir lacking EcoKI, the three type IV restriction systems, restored gentamicin sensitivity, harboring lacIq allele |
| Bartonella henselae |
|
|
|
B. henselae Houston-1 RSE247 |
rpsL |
our laboratory collection [60]; commonly used B. henselae laboratory strain |
| … MQ759 |
rpsL Δ(BH13960-BH13970)
|
our laboratory collection [64]; derivative of RSE247 deficient in production of BaGTA particles |
| … AHB0110 |
rpsL Δ(BH13960-BH13970) gyrA(G283A)
|
this study; ciprofloxacin-resistant derivative of MQ759 (carrying D95N mutation in GyrA) |
| … AHB0130 |
rpsL Δ(BH13960-BH13970) gyrA(G283A) CRAfT_v8.1
|
this study; pool of ca. 50 colonies from a transposition of CRAfT sensor transposon CRAfT_v8.1 into AHB0110 |
| … MFE137 |
rpsL ΔtrwE, ΔvirB2-11
|
our laboratory collection [65]; derivative of RSE247 deficient in VirB T4SS and Trw T4SS |
| … AHB0127 |
rpsL ΔtrwE, ΔvirB2-11 pVbh#2 pAH183_cre |
this study; derivative of MFE137 harboring pVbh#2 and a plasmid encoding the Cre control for CRAfT |
| … AHB0128 |
rpsL ΔtrwE, ΔvirB2-11 pVbh#2 pAH183_cre-traA |
this study; derivative of MFE137 harboring pVbh#2 and a plasmid encoding the Cre-TraA relaxase fusion for CRAfT |
| … AHB0129 |
rpsL ΔtrwE, ΔvirB2-11 pVbh#2 pAH183_cre-vbhT(H136A) |
this study; derivative of MFE137 harboring pVbh#2 and a plasmid encoding the Cre-VbhT(H136A) fusion for CRAfT |
| … PEE0403 |
rpsL ΔtrwE, ΔvirB2-11 pCD353 |
this study; derivative of MFE137 carrying pCD353 |
| Bartonella schoenbuchensis |
|
|
|
B. schoenbuchensis R1 CHDE366 |
pVbh |
our laboratory collection [58]; wildtype strain |
| … MLE0119 |
pVbh#2 |
this study; derivative of CHDE366 with gentamicin resistance cassette on pVbh (“pVbh#2”) |
| … PEE0352 |
rpsL; pVbh#2 |
this study; spontaneous streptomycin-resistant mutant of MLE0119 |
| … PEE0370 |
rpsL; pVbh#2 ΔvbhB4
|
this study; derivative of PEE0352 with scarless deletion of vbhB4
|
| … PEE0436 |
rpsL; pVbh#2 ΔtraA
|
this study; derivative of PEE0352 with scarless deletion of an N-terminal part of the TraA relaxase |
