Figure 3.

Keratinocyte Proliferation in Hair Folliculogenesis

(A) IVIS image from repTOPmitoIRE showing cell proliferation in animals treated with TRF or placebo (PBO) on days 7 and 21. (B) Immunohistochemical localization of Ki67 in paraffin sections of mouse skin showing the abundance of Ki67⁺ cells in mice treated (days 7 and 21) with TRF compared to PBO. Scale bars, 50 μm. Ki67⁺ cells were quantified and plotted graphically. Data are mean ± SD (n = 3) ^§p < 0.05; ^†p < 0.01. (C) Photomicrograph of H&E-stained paraffin sections showing epidermal thickening in mice treated (days 7 and 21) with TRF. The dermal and epidermal junctions are each marked by a dashed line. Scale bars, 20 μm. Epidermal thickness was quantified and plotted graphically. Data are mean ± SD (n = 4). *p < 0.001.