Figure 5.

Induction of β-Catenin and Nuclear Translocation

(A) TRF-induced (day 21) β-catenin (green) in murine epidermis. Counterstained with DAPI (blue). Scale bars, 50 μm. Fluorescence intensity was plotted graphically. Data are mean ± SD (n = 3). ^§p < 0.05. (B) Confocal microscopy showing TRF-induced translocation (day 21) of β-catenin into the nucleus. Counterstained with DAPI. Dermal (der) and epidermal junction indicated by a white dashed line. Scale bars, 10 μm. (C) Proximity ligation assay (PLA) showed β-catenin-Tcf3 co-localization in the nucleus in TRF treated adult skin. Scale bars, 10 μm. (D) Robust expression of SOX9, OCT4, K15, and K17 in TRF-treated (day 21) adult skin epidermis. The arrowhead indicates positive signals: red for DAB staining and white for fluorescent staining. Scale bars, 20 μm.