Table 1.

Specificity of Hu19-CD828Z

Effector T Cells	CD19-K562	Primary CLL	A549	MDA-MB231	293T	TC71	CCRF-CEM	T Cells Alonea
Hu19-CD828Z	29,198	3,922	<12	<12	26	26	171	101
Untransduced	431	123	<12	<12	143	28	21	<12

T cells transduced with LSIN-Hu19-CD828Z or left untransduced were cultured with the indicated target cells overnight, and a standard IFN-γ ELISA was performed. All values are in picograms per milliliter of IFN-γ. The CD19-K562 and primary CLL cells are CD19⁺ target cells. A549, MDA-MB231, 293T, TC71, and CCRF-CEM are CD19-negative target cells. Similar results were obtained in five experiments with cells from five different donors.

^aCAR T cells cultured without any target cells.