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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Angiogenesis. 2017 Aug 24;20(4):615–628. doi: 10.1007/s10456-017-9574-5

Figure 5. SFRP2 and Wnt5a bind FZD5.

Figure 5

Graphic representation of rhSFRP2 and rhWnt5a binding to FZD5-Fc showing changes in 450 nm optical density (OD450) as a function of the log of ligand concentrations. To do this, A microplate solid phase protein binding assay was performed using a concentration range of his-tagged recombinant human SFRP2 and Wnt5a from 24nM – 1500nM (x axis). The experiment was repeated twice for a total of n = 6. Kd, EC50 and Hill coefficient were calculated and compared for SFRP2 and Wnt5a binding to FZD5.