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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Angiogenesis. 2017 Aug 24;20(4):615–628. doi: 10.1007/s10456-017-9574-5

Figure 7. FZD5 is necessary for SFRP2 induced cell migration.

Figure 7

(A) 2H11 (1.5 × 104 cells), (B) HMEC-1 (2.5 × 104 cells), and (C) MDA-MB-231 cells (1.5 × 104 cells) control, treated with rhSFRP2 (30 nM), rhFZD5-Fc (500 ng/ml), or a combination of both were allowed to migrate for 24h in a trans-well assay. As depicted in the bar graphs (A–C), rhSFRP2 significantly promoted the migration of endothelial (A, B) and tumor cells (C) in similar proportions (~25%). rhFZD5-Fc alone had no significant effect on cell migration. However, when pre-incubated with rhSFRP2, rhFZD5-fc blunted the pro-migratory effects of rhSFRP2. Results represent the percent of migrating cells (+/− SEM), compared to control, and are the average of 3 independent experiments (n = 9). *: p<0.05.