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. 2017 Aug 24;25(11):2585–2598. doi: 10.1016/j.ymthe.2017.08.015

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

HDR Efficiency of the MC Double-Nicking Approach

96 subcloned nested PCR fragments were sequenced and analyzed. (A) Ratio between wild-type and mutated alleles. (B) Ratio between NHEJ (insertions and deletions) and HDR. n = absolute numbers of analyzed sequences. (C) HDR analysis. The position of sequence modifications (P) was defined according to the KRT14 target sequence exon 6-intron 6-exon 7-intron 7 (exon 6: P1-P221; intron 6: P222-P315; exon 7: P316-P362; intron 7: P363-P923). wRS, with restriction sites (exon 6: P1-P221; intron 6: P222-P315; exon 7: P316-P362; intron 7: P363-P935).