Table 1. Comparison of LM-based and EM measurements.
Bouton IDs match those in Figures 3 and 4. The probability that a putative bouton belongs to the category of LM boutons, was calculated according to Equation (9) with = 2.0. Bouton #8 (grey) was excluded from the analyses as it is a terminal bouton. Hyphens indicate that boutons, mitochondria, or PSDs were not detected in EM.
| 2PLSM measurements | EM measurements | |||||
|---|---|---|---|---|---|---|
| Bouton ID | Putative bouton weight, w | P(bouton | w) | Bouton volume [μm3] | Mitochondria volume [μm3] | PSD surface area [μm2] | |
| Axon 1 | 1 | 13.5 | 1.00 | 0.919 | 0.189 | 0.666 |
| 2 | 10.8 | 1.00 | 0.779 | 0.170 | 0.595 | |
| 3 | 1.98 | 0.48 | 0.093 | - | 0.371 | |
| 4 | 10.1 | 1.00 | 0.998 | 0.225 | 1.92 | |
| 5 | 8.65 | 1.00 | 0.669 | 0.139 | 1.83 | |
| 6 | 6.25 | 1.00 | 0.219 | 0.043 | 0.138 | |
| Axon 2 | 7 | 3.63 | 0.93 | 0.483 | 0.111 | 0.612 |
| 8 | N/A | N/A | 0.574 | - | 2.75 | |
| 9 | 5.57 | 1.00 | 0.372 | 0.027 | 1.28 | |
| Axon 3 | 10 | 9.54 | 1.00 | 0.632 | 0.182 | 0.645 |
| 11 | 1.99 | 0.49 | 0.102 | - | - | |
| 12 | 8.51 | 1.00 | 0.456 | - | 1.23 | |
| 13 | 10.8 | 1.00 | 0.704 | 0.161 | 1.49 | |
| Axon 4 | 14 | 5.80 | 1.00 | 0.308 | - | 0.867 |
| 15 | 4.23 | 1.00 | 0.198 | - | 0.686 | |
| 16 | 5.96 | 1.00 | 0.229 | 0.027 | 0.402 | |
| 17 | 2.85 | 0.93 | 0.140 | - | - | |
| 18 | 1.14 | 0.01 | - | - | - | |
| 19 | 2.19 | 0.65 | - | - | - | |