Figure 3.

Z-AAT Burden in NSG-PiZ Mice Decreases with Age and Hepatocyte Repopulation Correlates with Z-AAT Levels

(A) Correlation between the age of NSG-PiZ mice and their serum Z-AAT levels. Serum was collected and Z-AAT levels were quantified by ELISA. Data are plotted as mean ± SEM; n per group is as follows: 50 days, n = 10; 60 days, n = 9; 72 days, n = 27; 85 days, n = 12; 95 days, n = 17; 110 days, n = 7; 115 days, n = 3; 185 days, n = 5. (B–E) Liver tissue from NSG-PiZ mice was stained for PASD (B and C) or human AAT (D and E) at 6 weeks of age (B and D) or 14 weeks of age (C and E); results are presented for one representative animal. (F) Correlation between serum Z-AAT levels of the NSG-PiZ hosts at the time of engraftment and subsequent level of repopulation indicated by serum human albumin 7 weeks post-engraftment. Serum was collected at engraftment and 7 weeks post-engraftment, and Z-AAT and human albumin levels (respectively) were quantified by ELISA for 30 animals.