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. 2017 Jul 7;8(50):87107–87123. doi: 10.18632/oncotarget.19081

Figure 5. Inhibiting ERK1/2 activation by PD98059 suppresses the expression and translocation of Nrf2 protein.

Figure 5

Both MCF-7 and MDA-MB-231 cells were cultured without or with 100 μM propoxur, or 100 μM propoxur + 100 μM PD98059. (A) Representative blot and electrophoretogram of T-ERK1/2, p-ERK1/2 and Nrf2 proteins (B), respectively. (C) Representative confocal images of Nrf2 after different dosing of propoxur treatment (magnification, ×400). Following fixation, the cells were stained with a Nrf2 antibody (green) and DAPI (red).