Figure 4. WT1 activates the NAB2 promoter.

A reporter plasmid containing 1,300 bp NAB2 promoter sequence (Figure 3) was cotransfected by electroporation, with different amounts of expression plasmids encoding WT1+/−, WT1(delZ) or EGR1 into U937 cells, as indicated. Shown are normalized luciferase levels, relative to those obtained with promoter reporter only. Mean values, bars ±S.E.M., n = 4-7. Stars indicate statistical significance (^*: p < 0.05; ^**: p < 0.01; ^***: p < 0.001).