Skip to main content
. 2017 Aug 3;8(50):87136–87150. doi: 10.18632/oncotarget.19896

Figure 7. NAB2 counteracts WT1-mediated repression of the IRF8 promoter.

Figure 7

A reporter plasmid containing a 970 bp fragment of the IRF8 promoter [31] was transfected into 293T/17 together with an expression plasmid encoding WT1 and different amounts of NAB2 expression vector, as indicated. Fortyeight hours after transfection, cell lysates were collected and analyzed for luciferase activity. Shown are normalized firefly luciferase levels, relative to those obtained with promoter reporter only (control). Mean values, bars ±S.E.M., n = 3. Stars indicate statistical significance (*: p < 0.05; ***: p < 0.001). Western blot (WB) shows increasing amount of expressed NAB2. Histone H3 (H3) as equal loading control.