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. 2017 Aug 3;8(50):87136–87150. doi: 10.18632/oncotarget.19896

Figure 8. WT1 recruits NAB2 to the IRF8 promoter.

Figure 8

293T/17 cells were transfected with NAB2 (A, C) or with NAB2 plus WT1 (B, D). Nuclear extracts were obtained after 48 hours and cross-linked chromatin was prepared. (A, B) Chromatin immunoprecipitation (ChIP) using an antibody against NAB2 (Abcam, ab135665) or with anti-HA antibody as negative control was performed. PCR amplification of precipitated DNA was done with primers specific for the IRF8 promoter or with primers for the GAPDH promoter as negative control. Percentage of input, as determined by densitometry, shown as mean values, n = 3. (C, D) Fold enrichment as determined by densitometry. Mean values, bars ±S.E.M., n = 3. Star indicates statistical significance (*: p < 0.05), n.s.: not significant.