Table 1. Validation of the Cancer Hotspot Panel v2 by detecting hotspot mutations in four commonly mutated genes.
| Gene mutation; amino acid (Variant Allele Frequency) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell line | KRAS | TP53 | SMAD4 | CDKN2A | ||||||||
| 10 ng | 5 ng | 1 ng | 10 ng | 5 ng | 1 ng | 10 ng | 5 ng | 1 ng | 10 ng | 5 ng | 1 ng | |
| BxPC-3 | Wild Type | c.659A>G; Y220C (100%) | c.659A>G; Y220C (100%) | c.659A>G; Y220C (100%) | Homozygous deletion | Homozygous deletion | ||||||
| PANC-1 | c.35G>A; p.G12D (70.5%) | c.35G>A; p.G12D (70.2%) | c.35G>A; p.G12D (72.2%) | c.818G>A; R273H (100%) | c.818G>A; R273H (100%) | c.818G>A; R273H (100%) | Wild Type | Homozygous deletion | ||||
| MIA PaCa-2 | c.34G>T; p.G12C (100%) | c.34G>T; p.G12C (100%) | c.34G>T; p.G12C (100%) | c.742C>T; R248W (100%) | c.742C>T; R248W (100%) | c.742C>T; R248W (100%) | Wild Type | Homozygous deletion | ||||
Allele frequency shown in brackets where appropriate.