Table 3. Summary of the mutations identified in the cfDNA of PDAC patients by tNGS.
| Patient ID | cfDNA (ng/ml) | Gene | Mutation | Variant Allele Frequency (%) | Coverage | COSMIC | |
|---|---|---|---|---|---|---|---|
| Resectable PDAC | 63 | 540 | KRAS* | WT | -- | 606 | -- |
| 69 | 800 | KRAS* | WT | -- | 482 | -- | |
| 72 | 250 | KRAS | WT | -- | 507 | -- | |
| 92 | 220 | KRAS | WT | -- | 980 | -- | |
| 102 | 840 | KRAS* | WT | -- | 1448 | -- | |
| 104 | 450 | KRAS* | p.G12D | 8.7 | 473 | COSM521 | |
| Primary Non-Resectable PDAC | 16 | 232 | KRAS | WT | -- | 853 | -- |
| 19 | 300 | KRAS* | WT | -- | 2040 | -- | |
| 34 | 1190 | KRAS* | WT | -- | 1257 | -- | |
| 76** | 354 | KRAS | WT | na | na | na | |
| 80 | 610 | KRAS* | WT | -- | 464 | - | |
| Metastatic Non-Resectable PDAC | 12 | 120 | KRAS | WT | -- | 431 | -- |
| 13 | 270 | KRAS* | WT | -- | 456 | -- | |
| 14 | 710 | KRAS* | p.G12V | 1.4 | 1094 | COSM520 | |
| 15 | 700 | KRAS* | WT | -- | 2000 | -- | |
| 18 | 700 | KRAS* | p.G12R | 1.5 | 834 | COSM518 | |
| 21 | 2790 | KRAS* | p.G12R | 1.8 | 925 | COSM518 | |
| 24 | 300 | KRAS | WT | -- | 1155 | -- | |
| 26 | 4180 | KRAS* | WT | -- | 330 | -- | |
| 27 | 2620 | KRAS* | p.G12R | 62.9 | 705 | COSM518 | |
| 31 | 430 | KRAS* | p.G12V | 16.1 | 996 | COSM520 | |
| SMAD4 | p.P356R | 13.0 | 1642 | COSM339351 | |||
| TP53 | p.R248Q | 17.4 | 3857 | COSM10662 | |||
| 41 | 1150 | KRAS* | WT | -- | 388 | -- | |
| 53 | 970 | KRAS | p.G12R | 23.8 | 408 | COSM518 | |
| 68 | 280 | KRAS* | WT | -- | 1338 | -- | |
| 73 | 710 | KRAS* | WT | -- | 930 | -- | |
| 91 | 560 | KRAS* | WT | -- | 597 | -- |
--; not relevant as wild type, *; validated by ddPCR, **; identified by ddPCR validation, na; data not available.