Figure 1. Pan combination enhanced with lMB-100 activity synergistically.

KLM1 cells were treated with 20 nM of panbinostat, 10 ng/ml LMB-100 or both (Combination) and incubated for 3 days. The cells were all collected and stained with 7-AAD and Annexin-V. Typical staining profile were shown (A). (B) KLM1 cells were pretreated with 5 nM panbinostat for 6 hours (pretreated) or not pretreated, and then were treated with 20 nM panbinostst or LMB-100 5 ng/ml for 3 days. Dead cells include 7-AAD, Annexin V or both positive cells. The percentage of dead cells was calculated by subtracting the control dead cells. (C) and (D) KLM1 (C) or Panc3.014 cells (D) were treated as in (A). Dead cells were washed and fresh media were added to allow the live cells to recover. Cells were fixed and stained with crystal violet 4 days later.