Table 2. Apoptotic genes regulated by panbinostat.
| KLM1 | MKN28 | |||||
|---|---|---|---|---|---|---|
| Panbinostat | LMB-100 | Combination | Panbinostat | LMB-100 | Combination | |
| BNIP3L | 3.8 | 0.5 | 2.9 | 4.6 | 0.8 | 2.7 |
| CASP9 | 3 | 1.4 | 4.9 | 3.3 | 1.5 | 4.9 |
| CD40 | 4.2 | 0.8 | 5.1 | 14 | 0.9 | 21 |
| CD137 | 38 | 14 | 86 | 56 | 7.8 | 113 |
| CIAP2 | 5.5 | 5 | 17 | 5.3 | 4 | 12 |
| BID | 0.15 | 0.95 | 0.25 | 0.1 | 0.95 | 0.1 |
| cFLIP | 0.2 | 2.1 | 0.2 | 0.15 | 1.5 | 0.3 |
| NFκB1 | 0.2 | 2.7 | 0.4 | 0.2 | 1.8 | 0.5 |
| TP53 | 0.1 | 2 | 1.1 | 0.2 | 1 | 0.4 |
KLM1 or MKN28 cells in duplicate were pretreated with 5 nM panbinostat for 6 hours, then 20 nM panbinostat was added with or without 50 ng/ml LMB-100 for 18 hours. RNA were extracted and analyzed by apoptotic array (Qiagen). The numbers represent fold changes in RNA compared with control from an average of two samples. Genes that changed 3-fold or more by panbinostat, in both KLM1 and MKN28 cells, are presented here.