Table 2. PCR primers used in the gene expression analysis.
| Gene name | Description | Primer sequence (5′ - 3′) | Primer size (bp) |
|---|---|---|---|
| β-actin | mβ-actin-F | TTGCTGACAGGATGCAGAAG | 141 |
| mβ-actin-R | ACATCTGCTGGAAGGTGGAC | ||
| FSH-R | mFSHR-F | TTCTTGTGCCAATCCTTTCC | 108 |
| mFSHR-R | TAAATCTGGGCTTGCACCTC | ||
| LH-R | mLHR-F | ATGGCCATCCTCATCTTCAC | 146 |
| mLHR-R | TTGGCACAAGAATTGACAGG | ||
| INH-α | mINHα-F | GGTGGGGATCCTGGAATAAG | 122 |
| mINHα-R | GCACCTGTAGCTGGGAAAAG | ||
| INH-β B | mINHβB-F | TACGTGTGTCCAGAAGTGGC | 111 |
| mINHβB-R | TTCGCCTAGTGTGGGTCAAC | ||
| Ssty2 | mSsty2-F | AGGTCAACTGCCAACAAACC | 234 |
| mSsty2-R | ACCATCCCACTCCAGTTGTC | ||
| DDX3Y | mDDX3Y-F | CCGGCCTGGACCAGCAATTTGT | 239 |
| mDDX3Y-R | TGGGCTTCCCTCTGGAATCACGA | ||
| Sly | mSly-F | ACGAACGAGAGAGGAAGAACA | 210 |
| mSly-R | CCATGGACTTCTCATGCATTTC | ||
| HSF2 | mHSF2-F | AGGGGAGTACAACTGCATCG | 106 |
| mHSF2-R | TTACTCTGGGTCGGTTCTGG | ||
| StAR | mStAR-F | TGTCTCCCACTGCATAGCTG | 103 |
| mStAR-R | TGTTCGTAGCTGCTGGTGTC | ||
| P450scc | mP450scc-F | TTGGTTCCACTCCTCAAAGC | 127 |
| mP450scc-R | CCAAAGTCTTGGCTGGAATC | ||
| CYP17 | mCYP17-F | GAGGATCCATAGCGCAGAAG | 121 |
| mCYP17-R | GGGAGAGGAGAGGGAATTTG | ||
| CYP19 | mCYP19-F | CACATCATGCTGGACACCTC | 98 |
| mCYP19-R | TGCCAGGCGTTAAAGTAACC | ||
| 3β-HSD | m3β-HSD-F | TGCAGACAAAGACCAAGGTG | 106 |
| m3β-HSD-R | ACAGCAGCAGTGTGGATGAC | ||
| 17β-HSD | m17β-HSD-F | GATGTGGCTGTCAACTGTGC | 101 |
| m17β-HSD-R | TTGATAACCCGCTGGAAGTC |
Note: The primers were manufactured by Nanjing Genescript Co. Ltd. (Nanjing, P.R. China). 17β-HSD, 17-beta-hydroxysteroid dehydrogenase; 3β-HSD, 3-beta-hydroxysteroid dehydrogenase; CYP17, cytochrome P450c17 subfamily a; CYP19, cytochrome P450c19 subfamily a; FSH-R, follicle-stimulating hormone receptor; INH-α, inhibin subfamily α; INH-β B, inhibin subfamily β B; LH-R, luteinizing hormone receptor; P450scc, cholesterol side-chain cleavage enzyme; StAR, steroidogenic acute regulatory protein.