Skip to main content
. 2017 Sep 21;8(50):87675–87683. doi: 10.18632/oncotarget.21147

Figure 2. Cabazitaxel induced higher ROS accumulation than docetaxel in CRPC.

Figure 2

(A) Intracellular ROS level in C4-2AT6 cell was determined by fluorogenic marker carboxy-H2DCFCA. Fluorescence microscope detected elevated signals of GFP in C4-2AT6 cells treated with cabazitaxel (10 nM) than docetaxel (10 nM). CBZ: cabazitaxel, DOC: docetaxel. (B) Carboxy-H2DCFCA was quantified by FACS. FACS analysis revealed that treatment with docetaxel slightly increased ROS generation in C4-2AT6 cells. The fluorescent intensities were quantified by FACS. (C) Immunocytostaining showed significantly higher level of phosphor-p38MAPK in C4-2AT6 cell when treated with cabazitaxel, compared with docetaxel. (D) In C4-2AT6 cells treated with cabazitaxel (CBZ) or docetaxel (DOC), the mRNA expressions of MEKK4 (MAP3K4), MLK2, DLK1, MKK3(MAP2K3), MKK4(MAP2K4), ELK1 and MEF2C which were associated with ROS-mediated cellular signaling. * ; p< 0.05, ** ; p< 0.01, *** ; p< 0.01, compared with control.