Figure 3. Roles of elevated ROS production by cabazitaxel.

C4-2AT6 cells were treated with docetaxel (A) or cabazitaxel (B) in the presence or absence of antioxidant NAC for 24h and evaluated cell survival. NAC eliminated the cytotoxic effect of cabazitaxel in a dose dependent manner. In contrast, NAC didn't eliminated the cytotoxic effect of docetaxel. ^* ; p< 0.05, ^** ; p< 0.01, ^*** ; p< 0.01, compared with the viability at the same dose of docetaxel or cabazitaxel alone. The effect of ROS elimination by NAC in C4-2AT6 cells (C, D). Intracellular ROS level in C4-2AT6 cell was determined by fluorogenic marker carboxy-H₂DCFCA. The fluorescent intensities were quantified by FACS. Elevated expression of the cleaved-PARP after treatment with cabazitaxel was significantly attenuated by NAC (D). ^** ; p< 0.01, ^*** ; p< 0.01, compared with the viability at the same dose of cabazitaxel alone in the presence or absence of antioxidant NAC.