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. 2017 Sep 23;8(50):87718–87736. doi: 10.18632/oncotarget.21190

Figure 6. HADC-4 regulates claudin-2 expression in EGFR/ERK1/2-dependent manner.

Figure 6

(A-D) Immunoblot and densitometry analysis of EGFR, P-EGFR, P-ERK1/2 in total cell lysates of colon cancer cell treated with SAHA.; (E) Immunoblot analysis of EGFR and P-ERK1/2 using lysates prepared from control and HDAC-4 knockdown cells.; (F) Immunoblot analysis of claudin-2 and ERK-2(ERK2-MEK-1) in cells transfected with ERK2-MEK-1 construct and treated with or without SAHA.; (G) Representative co-immunoflurence staning of claudin-2 (green) and ZO-1(Red) in Caco-2 cells expressing constitutively active ERK-2 (ERK2-MEK-1 fusion protein) with or without SAHA-treatment. Three independent experiments were done and data is presented as mean ± S.E.M. *P<0.05, **P<0.01 and *** P<0.001 versus control.