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. 2017 Sep 23;8(50):87737–87749. doi: 10.18632/oncotarget.21209

Figure 4. SIRT7 is a direct target of miR-526b in HCC.

Figure 4

(A) The sequences of wild type (wt) or mutant (mt) SIRT7-3′UTR were cloned into the pmiR-RB-Report™ vector. (B) SMMC-7721 cells were transfected with miR-526b mimic or negative control NC) mimic and measured by qRT-PCR and immunoblotting for SIRT7. n = three repeats with similar results, *P<0.05 by t test. (C) miR-526b knockdown increased the level of SIRT7 in Hep3B cells. n = three repeats with similar results, *P<0.05 by t test. (D) Co-transfection of Hep3B cells with Pmir-RB-SIRT7 vector and miR-526b mimic or inhibitor significantly modulated luciferase reporter activity compared with the negative control. miR-526b mimic or inhibitor showed no significant effect on luciferase reporter activity of mt SIRT7-3′UTR. n = three repeats with similar results, *P<0.05 by t test.