Figure 9. SIRT7 restoration reversed miR-526b-induced downregulation of p-ERK and MET in SMMC-7721 cells.

miR-526b overexpressingSMMC-7721 cells that were transfected with SIRT7 vector or empty vector (EV) were detected by immunoblotting for p-ERK, E-cadherin and Vimentin. Re-expression of SIRT7 significantly increased the levels of p-ERK and Vimentin, and reduced E-cadherin expression in miR-526b overexpressing SMMC-7721 cells. n = three repeats with similar results, ^*P<0.05 by t test.