Figure 2. Minocycline extends lifespan in w¹¹¹⁸ Drosophila strain.

Minocycline treatment (0.05 mM and 0.36 mM) extends lifespan of w¹¹¹⁸ flies, as shown by the survival curve (left) and by quantification of median lifespan (right). A more pronounced lifespan extension was induced by the low dose. The longevity effect of the drug was seen in both male (upper panel) and female (lower panel) flies. For male flies, n = 228 (normal), n = 230 (0.05 mM), n = 220 (0.36 mM); p < 1.0 × 10⁻¹⁰ (normal vs. 0.05 mM), p = 0.0039 (normal vs. 0.36 mM). For female flies, n = 225 (normal), n = 232 (0.05 mM), n = 220 (0.36 mM); p < 1.0 × 10⁻¹⁰ (both normal vs. 0.05 mM and normal vs. 0.36 mM). p-values for lifespan curves were calculated by the log-rank test. All lifespan experiments were repeated with similar results; representative experiments are shown. Data for median lifespan are presented as mean ± SEM.