Figure 5. Minocycline treatment increases resistance to oxidative stress.
Flies fed minocycline showed a greater resistance to hydrogen peroxide than control flies. This phenomenon was observed in both male (upper panel) and female (lower panel) flies. A high dose (0.36 mM) of minocycline appears to confer greater resistance to oxidative stress. For male flies, n = 101 (normal), n = 119 (0.05 mM), n = 105 (0.36 mM); p = 3.7 × 10−7 (normal vs. 0.05 mM), p = 5.9 × 10−7 (normal vs. 0.36 mM). For female flies, n = 101 (normal), n = 115 (0.05 mM), n = 116 (0.36 mM); p = 0.0004 (normal vs. 0.05 mM), p < 1.0 × 10−10 (normal vs. 0.36 mM). p-values for lifespan curves were calculated by the log-rank test. All lifespan experiments were repeated with similar results; representative experiments are shown.