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. 2017 Sep 23;8(50):87878–87890. doi: 10.18632/oncotarget.21224

Figure 7. Minocycline treatment increase FOXO activity.

Figure 7

(A) Minocycline treatment for 3 days frequently increases mRNA expression of FOXO and its target genes such as InR and 4E-BP. This phenotype is broadly seen across the tissues including head (left panel), thoracic muscle (middle panel), and abdominal fat body tissues (right panel), and is also consistently seen in both male (♂) and female (♀) flies. n=18 (head), n=10 (thoracic muscle), and n=10 (abdominal fat body) per treatment. (B) Representation of minocycline's influence on mRNA expression of FOXO, InR and 4E-BP by pooling individual data sets separated by tissues and sexes shown in panel A. (C) Minocycline treatment increases the expression of FOXO protein but marginally affects Akt and JNK activity shown by western blot (left panel) and its quantification (right panel). Flies were treated with hydrogen peroxide and indicated concentrations of minocycline for 2 days and abdominal fat bodies were prepared. Similar results were seen in head and thoracic muscle tissues. Three biological replicates were analyzed. Data are presented as mean ± SEM. *p <0.05 when compared to the respective controls (t-test). n.s., not significant.