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. 2017 Sep 30;8(50):88043–88058. doi: 10.18632/oncotarget.21408

Figure 5. Investigation of possible signaling pathway related to TMEM98 in IL-8-treated vascular wall cells.

Figure 5

Real time RT-PCR analysis of the expression ICAM-1 after transfection of siRNA-TMEM98 into HUVEC cells (A) Western blot confirmed the protein level of AKT and EKR1/2 after transfection of siRNA-TMEM98 into AoSMC (B) The graphic showed the statistical data of Figure 5B (C and D). Western blot confirmed the protein level of p-PI3K, PI3K p85 (Y607), p-AKT (S473), p-AKT (T308), t-AKT, p-GSK-3β, p-c-Raf (S259), p-ERK1/2 and t-ERK1/2 after transfection of siRNA-TMEM98 into A7r5 (E) The graphic showed the statistical data of Figure 5E (F) Cyclin D1 was examined by Western blot (G and H). All experiments were performed in biologic triplicates. And the data were shown as the mean ± SD. *p<0.05, **p<0.01.