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. 2017 Oct 4;8(50):88163–88178. doi: 10.18632/oncotarget.21513

Figure 4. CRNDE overexpression promotes proliferation, migration, and invasion, and inhibits apoptosis in glioma cells.

Figure 4

(A) Increased CRNDE levels in U251 cells after transfection with pEX-2-CRNDE. Data are presented as mean ± SD (n = 3, each group). **P< 0.01 vs. NT group. ## P < 0.01 vs. pEX-2-NC group. (B) Effects of transfection with pEX-2-CRNDE or pEX-2-NC on the proliferation of U251 cells. (C, D) The wound healing assay was used to assess migration capacity in U251 cells transfected with pEX-2-CRNDE or pEX-2-NC. Wound closure was measured at 24 and 48 h. Representative images and accompanying statistical plots are presented. Data are presented as mean ± SD (n = 3, each group). **P< 0.01 vs. NT group. ## P < 0.01 vs. pEX-2-NC group. Scale bars represent 100μm. (E, F) Matrigel invasion assay in U251 cells transfected with pEX-2-CRNDE or pEX-2-NC. Representative images and accompanying statistical plots are presented. Data are presented as mean ± SD (n = 5, each group). **P< 0.01 vs. NT group. ## P< 0.01 vs. pEX-2-NC group. Scale bars represent 50μm. (G, H) Apoptosis assay by flow cytometry inU251 cells transfected with pEX-2-CRNDE or pEX-2-NC. Representative images and accompanying statistical plots are presented. Data are presented as mean ± SD (n = 3, each group). **P< 0.01 vs. NT group. ## P < 0.01 vs. pEX-2-NC group. NT, non-transfected cells. NC, negative control. pEX-2-NC, pEX2 negative control plasmid; pEX-2-CRNDE, CRNDE full length plasmid.