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. Author manuscript; available in PMC: 2017 Nov 7.
Published in final edited form as: Nature. 2017 Aug 30;549(7670):111–115. doi: 10.1038/nature23875

Extended Data Figure 5. Chromatin features and enhancer activity of IL2RA CaREs.

Extended Data Figure 5

a, Results of the IL2RA CRISPRa screen are overlapped with with DNase I hypersensitivity, H3K27ac, and H3K4me1 datasets from primary human haematopoietic cell types. Data from the Roadmap Epigenomics Project. Jurkat DNase HS data are from ENCODE. b, Jurkat cells were nucleofected with luciferase reporter constructs containing IL2RA CaRE sequences upstream of a generic minimal promoter. 18 h after nucleofection, cells were split to a stimulation plate coated with anti-CD3/CD28 or a PBS control plate. Cells were lysed 24 h later and luciferase activity was measured. Data are presented as mean ± s.d., n = 4 biological replicates, representative of two independent experiments. Dotted line represents relevant luciferase activity defined as two times activity of sequence-scrambled IL2RA CaRE4 control. **** P ≤ 0.0001 by one-way ANOVA followed by Dunnet’s multiple comparisons test.