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. Author manuscript; available in PMC: 2018 Nov 10.
Published in final edited form as: J Chromatogr A. 2017 Aug 15;1523:114–122. doi: 10.1016/j.chroma.2017.08.032

Figure 5.

Figure 5

Electropherograms obtained for AGP glycoforms when using various samples and sample pretreatment methods, including 2.0 mg/L AGP in water (i.e., which was used as a positive control), serum that was processed by acid precipitation and desalting, serum that was processed by desalting alone, and serum that was used with no pretreatment (as a control). Conditions: separation voltage, −30 kV; capillary temperature, 25 °C; running buffer, 20 mM acetate buffer (pH 4.2) containing 0.05% PEO and 0.1% Brij 35; and injection conditions, −5 kV for 5 min. The capillary coating procedure is described in Section 2.2.