Table 2.
Conductivity and resistivity measurements for samples after various pretreatment stepsa
| Sample or solution | Conductivity (μS/cm) | Resistivity ratio (γ) vs. running buffer |
|---|---|---|
| Serum with no pretreatmentb | 147.5 (± 0.2) | 2.59 (± 0.01) |
| Serum after desaltingb | 20.95 (± 0.03) | 18.25 (± 0.04) |
| Serum after acid precipitation and desaltingb | 12.96 (± 0.05) | 29.5 (± 0.1) |
| Sample of 2 g/L AGP in water | 6.76 (± 0.04) | 56.6 (± 0.3) |
| Running bufferc | 382.4 (± 0.6) | n/a |
a
The numbers in the parentheses represents a range of ±1 S.D.
b
The serum sample was diluted by 115.4-fold after each of these pretreatment procedures.
c
The running buffer was pH 4.2, 20 mM acetate buffer that contained 0.05% (w/v) PEO and 0.1% (w/v) Brij 35.