Table 3.
Characteristics of an electrophoretic injection CE method for AGP glycoform analysis in serum using acid precipitation and desalting for sample pretreatment
| Peak | Limit of detection (nM)a | Plate number (×103)b | Precision of migration timeb | Precision of peak areab | Recoveryc |
|---|---|---|---|---|---|
| 3 | 2.5 | 8.11 (± 0.37) | ± 0.08% | 0.86% | 72.3% |
| 4 | 8.2 | 7.12 (± 0.08) | ± 0.11% | 0.94% | 80.9% |
| 5 | 11.4 | 5.34 (± 0.10) | ± 0.12% | 0.71% | 80.4% |
| 6 | 8.5 | 4.32 (± 0.06) | ± 0.12% | 0.52% | 80.5% |
| 7 | 4.8 | 4.10 (± 0.03) | ± 0.12% | 0.34% | 80.3% |
| 8 | 2.4 | 3.87 (± 0.02) | ± 0.13% | 0.86% | 78.2% |
| 9 | 2.1 | 2.84 (± 0.02) | ± 0.12% | 1.18% | 73.6% |
The limit of detection was calculated by using a signal-to-noise ratio of three, as based on the standard deviation of the intercept and the slope from a calibration curve for this assay.
The precisions and plate numbers are based on triplicate measurements for normal serum that was spiked with 2.5 g/L AGP. All numbers in parentheses represent ± 1 S.D. The precisions for the migration times and peaks areas are given in terms of ± 1 relative standard deviation.
The recovery was calculated by dividing the slopes of the corresponding calibration curves that were obtained before and after sample pretreatment with pooled normal serum that had been spiked with a known amount of AGP. Each sample was analyzed by CE in triplicate.