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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Cancer Lett. 2017 Sep 29;410:191–200. doi: 10.1016/j.canlet.2017.09.038

Figure 3. Aberrant methylation of ANK1 promoter CpG islands in lung cancer.

Figure 3

Methylation of (A) ANK1B and (B) ANK1E promoter CpG islands in normal (top panels) and lung cancer (middle and bottom panels) was evaluated using the semi-quantitative Combined Bisulfite Modification and Restriction Analysis (CoBRA). Partial or complete digestion of PCR products into smaller fragments following the addition (+) of the BstU1 restriction enzyme indicates partial or complete methylation. Partial demethylation of the two promoter CpG islands (bottom panels) following treatment with the methylation inhibitor DAC compared to control shown by the re-appearance of some undigested bands, indicates reversibility of these epigenetic changes.