Skip to main content
. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: J Immunol. 2017 Oct 9;199(10):3437–3452. doi: 10.4049/jimmunol.1700953

Figure 7. Analysis of TRAV29-mediated functional response to Gag293 stimulation.

Figure 7

(A) A highly prevalent TRAV29 clonotype was paired with two TRBV2 clonotypes derived from the ADVAX-induced Gag293-specific repertoire, to generate the AS1 and AS7 TCRs. F24, a high-avidity TCR isolated from HIV controllers was used as a positive control. The F24 TCR comprised the most prevalent TRAV24 public clonotype paired to one TRBV2 public clonotype.

(B to D) The AS1 and AS7 TCRs were expressed in Jurkat J76 cells, which lack an endogenous TCR complex. Transduced J76 cell were stimulated with APCs expressing human DR1 (B), DR11 (C), DR15 (D), or DRB5 (E) pulsed with decreasing doses of Gag293 peptide. T cell activation was measured by the expression of CD69 following overnight stimulation. One representative experiment out of three is depicted.