Figure 1.

Reduced NK cell surface expression of NK1.1 and Ly49D in mice with enforced ligand-induced NKG2D downregulation. Numeric and phenotypic assessment of NK cells from H2-K^b-MICA mice by flow cytometry. Splenic NK cells were isolated either from naïve mice or 16 h after poly(I:C) injection (activated NK cells). (A,B) No significant differences in frequencies (A) or total numbers (B) between resting or activated splenic NK cells from H2-K^b-MICA mice and non-transgenic littermates (non-tgLM). NK cells were gated as NK1.1⁺CD3⁻ splenocytes, and in panel (A) also as NKp46⁺CD3⁻ splenocytes. Data show means plus SE from three mice analyzed in parallel. One representative out of at least three independent experiments is shown. A two-way ANOVA was performed with a Sidak posttest. (C) Surface expression of activating receptors NKG2D, NKp46, Ly49D, Ly49H, NK1.1, and CD16 on splenic NK cells from naïve or poly(I:C) injected H2-K^b-MICA mice versus non-tgLM. Data show means plus SDs from three mice analyzed in parallel. One representative out of at least three independent experiments is shown. Two-way ANOVA was performed with a Tukey posttest to assess statistical significance of differences (ns = not significant).